CD300a antibody (APC)

Catalog No. ABIN6240821

This Mouse Monoclonal antibody specifically detects CD300a in FACS. It exhibits reactivity toward Human.

Quick Overview for CD300a antibody (APC) (ABIN6240821)

Target: CD300a (CD300A)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD300a antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: MEM-260

Product Details anti-CD300a Antibody (APC)

Purpose: Anti-Hu CD300a APC

Specificity: The antibody MEM-260 reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25 % of circulating T cells and 15 % of circulating B cells.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: HPB human acute lymphoid leukemia cell line

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for CD300a

Target: CD300a (CD300A)

Alternative Name: CD300a

Background: CD300a molecule,CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.,IRC, CLM-8, IGSF12, CMRF35H

Gene ID: 11314

UniProt: Q9UGN4

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process