HLA-A+B+C+E antibody (PE)

Catalog No. ABIN6240830

This Mouse Monoclonal antibody specifically detects HLA-A+B+C+E in FACS. It exhibits reactivity toward Human.

Quick Overview for HLA-A+B+C+E antibody (PE) (ABIN6240830)

Target: HLA-A+B+C+E

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS)

Clone: TP25-99SF

Product Details

Purpose: Anti-HLA-ABCE PE

Specificity: The mouse monoclonal antibody TP25.99SF recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: IFN-gamma-treated human melanoma cells Colo 38

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details

Target: HLA-A+B+C+E

Alternative Name: HLA-ABCE

Background: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.