Ubiquitin B antibody (N-Term)

Catalog No. ABIN6242685

The Rabbit Polyclonal anti-Ubiquitin B antibody has been validated for WB. It is suitable to detect Ubiquitin B in samples from Human, Mouse and Rat.

Quick Overview for Ubiquitin B antibody (N-Term) (ABIN6242685)

Target: Ubiquitin B (UBB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin B antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB49507

Product Details anti-Ubiquitin B Antibody

Binding Specificity: AA 25-57, N-Term

Predicted Reactivity: B, E, D, Pr, Pig, Sh, C, Hs, Ha, X, Rb

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This UBB(Ubiquitin) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57amino acids from the N-terminal region of human UBB(Ubiquitin).

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for Ubiquitin B

Target: Ubiquitin B (UBB)

Alternative Name: UBB(Ubiquitin)

Background: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Molecular Weight: 25762

UniProt: P0CG47

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway