DIAPH1 antibody (N-Term)

Catalog No. ABIN6242839

This anti-DIAPH1 antibody is a Rabbit Polyclonal antibody detecting DIAPH1 in WB. Suitable for Human.

Quick Overview for DIAPH1 antibody (N-Term) (ABIN6242839)

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIAPH1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB30760

Product Details anti-DIAPH1 Antibody

Binding Specificity: AA 18-52, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DIAPH1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 18-52 amino acids from the N-terminal region of human DIAPH1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for DIAPH1

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Alternative Name: DIAPH1

Background: Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA- DIAPH1 signaling pathway plays an important role in ERBB2- dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.

Molecular Weight: 141347

UniProt: O60610

Pathways: Sensory Perception of Sound