Nanog antibody (AA 155-189)

Catalog No. ABIN6242860

This anti-Nanog antibody is a Rabbit Polyclonal antibody detecting Nanog in WB. Suitable for Human.

Quick Overview for Nanog antibody (AA 155-189) (ABIN6242860)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB52819

Product Details anti-Nanog Antibody

Binding Specificity: AA 155-189

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NANOG antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 155-189 amino acids from the Central region of human NANOG.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Molecular Weight: 34620

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance