RBCK1 antibody

Catalog No. ABIN6243298

The Mouse Monoclonal anti-RBCK1 antibody (Clone 1839CT718-19-54) (ABIN6243298) specifically detects RBCK1 in WB. The antibody is reactive with Human samples.

Quick Overview for RBCK1 antibody (ABIN6243298)

Target: RBCK1 (RanBP-Type and C3HC4-Type Zinc Finger Containing 1 (RBCK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1839CT718-19-54

Product Details anti-RBCK1 Antibody

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This RBCK1 (UBCE7IP3) antibody is generated from a mouse immunized with a recombinant protein of human RBCK1 (UBCE7IP3).

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for RBCK1

Target: RBCK1 (RanBP-Type and C3HC4-Type Zinc Finger Containing 1 (RBCK1))

Alternative Name: RBCK1 (UBCE7IP3)

Background: E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF- kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF- RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types.

Molecular Weight: 57572

UniProt: Q9BYM8