Nucleoli Marker antibody

Catalog No. ABIN6251821

The Mouse Monoclonal anti-Nucleoli Marker antibody is suitable to detect Nucleoli Marker in samples from Human. It has been validated for IF, FACS, IHC and ICC.

Quick Overview for Nucleoli Marker antibody (ABIN6251821)

Target: Nucleoli Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: NCM102-1

Product Details

Purification: Protein G affinity chromatography

Immunogen: A preparation of myeloid leukemia cell nuclei was used as the immunogen.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 0.5-1.0 μg/mL

Immunocytochemistry (Acetone-fixed): 0.5-1.0 μg/mL for 30 min at RT

IHC (Formalin-paraffin): 0.5-1.0 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the Nucleoli marker antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details

Target: Nucleoli Marker

Background: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]