TFF1 antibody (C-Term)

Catalog No. ABIN6251839

The Mouse Monoclonal anti-TFF1 antibody has been validated for WB, IHC, IF and FACS. It is suitable to detect TFF1 in samples from Human and Cynomolgus.

Quick Overview for TFF1 antibody (C-Term) (ABIN6251839)

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reactivity: Human, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: GE2

Product Details anti-TFF1 Antibody

Binding Specificity: C-Term

Purification: Protein G affinity chromatography

Immunogen: Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (57-84) from the C-terminus of human Estrogen inducible protein pS2 was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues REQUIRES digestion with Pronase E at 1 mg/mL in PBS for 15 min at 37 °C. Heat Induced Epitope Retrieval is not suitable for this antibody.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: Estrogen Inducible Protein pS2

Background: This antibody recognizes a polypeptide of ~6.5  kDa, identified as Estrogen Inducible Protein pS2. The antibody's epitope is localized between amino acids 57-84. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. Estrogen Inducible Protein pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60 % of breast carcinomas are positive for estrogen inducible protein pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that estrogen inducible protein pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

Gene ID: 7031

UniProt: P04155

Pathways: EGFR Signaling Pathway