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Ferritin antibody (AA 38-165)

This Mouse Monoclonal antibody specifically detects Ferritin in WB, IHC, IF and FACS. It exhibits reactivity toward Human.
Catalog No. ABIN6252003

Quick Overview for Ferritin antibody (AA 38-165) (ABIN6252003)

Target

See all Ferritin (FE) Antibodies
Ferritin (FE)

Reactivity

  • 72
  • 13
  • 13
  • 8
  • 7
  • 1
  • 1
Human

Host

  • 50
  • 32
  • 2
  • 2
Mouse

Clonality

  • 59
  • 26
Monoclonal

Conjugate

  • 53
  • 11
  • 5
  • 5
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Ferritin antibody is un-conjugated

Application

  • 48
  • 30
  • 21
  • 15
  • 10
  • 8
  • 7
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone

FTLC1-1
  • Binding Specificity

    • 8
    • 2
    • 1
    • 1
    • 1
    AA 38-165

    Purification

    Protein G affinity chromatography

    Immunogen

    Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin antibody (light chain specific).

    Isotype

    IgG2b
  • Application Notes

    Western blot: 1-2 μg/mL

    Flow Cytometry: 0.5-1 μg/10^6 cells

    IF: 1-2 μg/mL

    IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.2 mg/mL

    Buffer

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target

    Ferritin (FE)

    Alternative Name

    Ferritin

    Background

    Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

    Gene ID

    2512

    UniProt

    P02792

    Pathways

    Transition Metal Ion Homeostasis
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