Involucrin antibody (AA 421-568)

Catalog No. ABIN6252058

The Mouse Monoclonal anti-Involucrin antibody has been validated for IHC, IF and FACS. It is suitable to detect Involucrin in samples from Human, Dog, Pig, Gorilla and Owl Monkey.

Quick Overview for Involucrin antibody (AA 421-568) (ABIN6252058)

Target: Involucrin (IVL)

Reactivity: Human, Dog, Pig, Gorilla, Owl Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Involucrin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: SPM259

Product Details anti-Involucrin Antibody

Binding Specificity: AA 421-568

Specificity: Does not react with Mouse

Purification: Protein G affinity chromatography

Immunogen: Purified involucrin from human keratinocytes was used as the immunogen for this Involucrin antibody. Its epitope maps between amino acids 421-568 of human involucrin.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The optimal dilution of the Involucrin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37oC. Enzyme digestion is better than citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Involucrin

Target: Involucrin (IVL)

Alternative Name: Involucrin

Background: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Gene ID: 3713

UniProt: P07476