Nucleolin antibody

Catalog No. ABIN6252098

This anti-Nucleolin antibody is a Mouse Monoclonal antibody detecting Nucleolin in WB, IF, FACS and IHC (p). Suitable for Human and Mouse.

Quick Overview for Nucleolin antibody (ABIN6252098)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NPC23-2

Product Details anti-Nucleolin Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Isotype: IgG1

Application Details

Application Notes: IF: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Western blot: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the Nucleolin antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization