Fibronectin antibody

Catalog No. ABIN6252186

This Mouse Monoclonal antibody specifically detects Fibronectin in IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Fibronectin antibody (ABIN6252186)

Target: Fibronectin

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: HFN7-1

Product Details anti-Fibronectin Antibody

Purification: Protein G affinity chromatography

Immunogen: Purified human Fibronectin was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Titration of the antibody may be required for optimal performance.

1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Fibronectin

Target: Fibronectin

Background: The Fibronectin dimer consists of two disulfide linked monomers of approximately 220 kD, and is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. It accumulates at the site of injury and, along with fibrin, forms a blood clot to stop bleeding and protect damaged tissue.

The HFN7.1 Fibronectin antibody is directed against the peptide core and reacts with both the plasma and cellular forms. The antibody blocks the fibronectin-mediated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.

Gene ID: 2335

UniProt: P02751