Fibronectin antibody

Catalog No. ABIN6252187

The Mouse Monoclonal anti-Fibronectin antibody has been validated for IHC, IF and FACS. It is suitable to detect Fibronectin in samples from Human, Rat, Mouse and Pig.

Quick Overview for Fibronectin antibody (ABIN6252187)

Target: Fibronectin

Reactivity: Human, Rat, Mouse, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: SPM246

Product Details anti-Fibronectin Antibody

Purification: Protein G affinity chromatography

Immunogen: A T-lymphoma biopsy was used as the immunogen for this Fibronectin antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

The optimal dilution of the Fibronectin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectin is a soluble dimeric glycoprotein of 440  kDa, which is present in cells, extracellular matrix, and blood. This mAb reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this mAb localizes to tumor vessels where it binds to the underlying basement. Epitope recognized by this antibody is not accessible in normal tissues to the circulating mAb indicating that it can be used to specifically target tumor vessels in vivo.

Gene ID: 2335

UniProt: P02751