ICAM-3/CD50 antibody

Catalog No. ABIN6252201

This Mouse Monoclonal antibody specifically detects ICAM-3/CD50 in FACS, IF, IHC and BI. It exhibits reactivity toward Human.

Quick Overview for ICAM-3/CD50 antibody (ABIN6252201)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM-3/CD50 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), BioImaging (BI)

Clone: 186-2G9

Product Details anti-ICAM-3/CD50 Antibody

Purification: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as the immunogen for this CD50 antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Functional Activity (Blocking): Blocks CD50-CD11a interaction

Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD50 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: CD50

Background: This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120  kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI). It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.

Gene ID: 3385

UniProt: P32942