Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Intercellular Adhesion Molecule 3 (ICAM3) antibody (Biotin) Primary Antibody

ICAM3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-171 Biotin
Pubmed (3)
Catalog No. ABIN94168
$455.40
Plus shipping costs $45.00
0.1 mg
local_shipping Shipping to: United States
Delivery in 5 to 7 Business Days
  • Target
    Intercellular Adhesion Molecule 3 (ICAM3)
    Reactivity
    Human
    • 175
    • 3
    • 2
    • 2
    Host
    Mouse
    • 128
    • 45
    • 1
    Clonality
    Monoclonal
    Conjugate
    Biotin
    • 76
    • 11
    • 11
    • 10
    • 7
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Application
    Flow Cytometry (FACS), Immunoprecipitation (IP)
    • 116
    • 82
    • 71
    • 57
    • 45
    • 42
    • 31
    • 22
    • 8
    • 7
    • 7
    • 6
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Specificity
    The antibody MEM-171 recognizes an epitope in the D2 domain of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells, it is negative on platelets and erythrocytes.
    HLDA VI, WS Code BP 614
    HLDA VI, WS Code NL N-L022
    Cross-Reactivity (Details)
    Human
    Immunogen
    Human granulocytes
    Clone
    MEM-171
    Isotype
    IgG1
  • Application Notes
    Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
    Comment

    The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Linnebacher, Wienck, Boeck, Klar: "Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO)." in: Journal of biomedicine & biotechnology, Vol. 2010, pp. 841451, 2010 (PubMed).

    Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, 2007 (PubMed).

    Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, 2002 (PubMed).

  • Target
    Intercellular Adhesion Molecule 3 (ICAM3)
    Alternative Name
    CD50 (ICAM3 Antibody Abstract)
    Synonyms
    CD50, CDW50, ICAM-R, ICAM-3, ICAM3, intercellular adhesion molecule 3, ICAM3, Icam3
    Background
    CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages, this process is involved in dialogue between dendritic cells and granulocytes.
You are here:
help Support