Intercellular Adhesion Molecule 3 (ICAM3) antibody (Biotin)

Details for Product No. ABIN94168
Antigen
Reactivity
Human
309
5
5
3
2
Host
Mouse
227
80
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
18
15
14
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
188
144
109
81
78
68
51
48
14
11
10
9
8
6
6
4
4
3
2
2
1
1
Options
Immunogen Human granulocytes
Clone MEM-171
Isotype IgG1
Specificity The antibody MEM-171 recognizes an epitope in the D2 domain of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells, it is negative on platelets and erythrocytes.
HLDA VI, WS Code BP 614
HLDA VI, WS Code NL N-L022
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD50 (ICAM3 Antibody Abstract)
Background CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages, this process is involved in dialogue between dendritic cells and granulocytes.
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (Biotin) (ABIN94168) Surface staining of JURKAT humanleukemia T cell line with anti-human CD50 (MEM-171) F...
Product cited in: Linnebacher, Wienck, Boeck, Klar: "Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO)." in: Journal of biomedicine & biotechnology, Vol. 2010, pp. 841451, 2010 (PubMed).

Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, 2007 (PubMed).

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, 2002 (PubMed).