Melanoma antibody
Quick Overview for Melanoma antibody (ABIN6252230)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- Does not react with rat
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Purification
- Protein G affinity chromatography
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Immunogen
- Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
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Isotype
- IgG2b kappa
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anti-Melanoma antibody
Reactivity: Human, Dog, Mouse IHC (p), IF Host: Mouse Monoclonal PNL2 unconjugated
anti-Melanoma antibodyReactivity: Human, Horse IHC (p), IF, FACS Host: Mouse Monoclonal NKI-beteb unconjugated
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Application Notes
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Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.2 mg/mL
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Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Melanoma
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Background
- By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
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Gene ID
- 6490
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UniProt
- P40967
Target
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