Melanoma antibody

Catalog No. ABIN3024167

Product Details anti-Melanoma Antibody

Target: Melanoma

Reactivity: Human, Dog, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Characteristics: Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87 % ), as opposed to 76 % for anti-HMB45 and 82 % for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF.

Purity: Protein G affinity chromatography

Immunogen: Melanocyte antigen was used as the immunogen for the Melanoma antibody PNL2.

Clone: PNL2

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the Melanoma antibody PNL2 should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in sterile PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C,4 °C

Storage Comment: Store the Melanoma antibody PNL2 at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Melanoma

Target: Melanoma

Abstract: Melanoma Products