VEGF antibody

Catalog No. ABIN6252287

This anti-VEGF antibody is a Mouse Monoclonal antibody detecting VEGF in WB, IHC (p) and IF. Suitable for Human, Mouse, Rat, Dog and Rabbit.

Quick Overview for VEGF antibody (ABIN6252287)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: VEGF-1063

Product Details anti-VEGF Antibody

Purification: Protein G affinity chromatography

Immunogen: Human recombinant protein was used as the immunogen for the VEGF antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Optimal dilution of the VEGF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF

Background: This mAb recognizes proteins of 19-22  kDa (reducing) and 38  kDa-44  kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Gene ID: 7422

UniProt: P15692