KRT14 antibody (C-Term)

Catalog No. ABIN6252305

This anti-KRT14 antibody is a Mouse Monoclonal antibody detecting KRT14 in WB, IHC, IF and FACS. Suitable for Human, Rat and Mouse.

Quick Overview for KRT14 antibody (C-Term) (ABIN6252305)

Target: KRT14 (Keratin 14 (KRT14))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: LL002

Product Details anti-KRT14 Antibody

Binding Specificity: C-Term

Purification: Protein G affinity chromatography

Immunogen: A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14 was used as the immunogen.

Isotype: IgG3

Application Details

Application Notes: Western blot: 0.5-1 μg/mL

Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Cytokeratin 14

Background: Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). Cytokeratin 14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. antibody to cytokeratin 14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. cytokeratin 14 antibody is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Cytokeratin 14 antibody is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Gene ID: 3861

UniProt: P02533