FLI1 antibody (AA 107-216)

Catalog No. ABIN6252309

This Mouse Monoclonal antibody specifically detects FLI1 in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for FLI1 antibody (AA 107-216) (ABIN6252309)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: FLVI1-1

Product Details anti-FLI1 Antibody

Binding Specificity: AA 107-216

Purification: Protein G affinity chromatography

Immunogen: Human partial recombinant protein (amino acids 107-216) was used as the immunogen for this FLI1 antibody.

Isotype: IgG

Application Details

Application Notes: Western blot: 1-2 μg/m,l

Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FLI1 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: FLI1

Background: Friend leukemia integration 1 is a member of the ETS family of DNA binding transcription factors that is involved in cellular proliferation and tumorigenesis. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90 % of Ewing's Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

Gene ID: 2313

UniProt: Q01543