LAMP3 antibody

Catalog No. ABIN6252513

This Mouse Monoclonal antibody specifically detects LAMP3 in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for LAMP3 antibody (ABIN6252513)

Target: LAMP3 (Lysosomal-Associated Membrane Protein 3 (LAMP3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAMP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: LAMP3-529

Product Details anti-LAMP3 Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the LAMP3 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Optimal dilution of the LAMP3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for LAMP3

Target: LAMP3 (Lysosomal-Associated Membrane Protein 3 (LAMP3))

Alternative Name: LAMP3

Background: This mAb recognizes protein of 26  kDa-60  kDa, which is identified as CD63/LAMP3. Its epitope is different from that of mAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63/LAMP3 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. It is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63/LAMP3 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Gene ID: 967

UniProt: P08962