ECM1 antibody

Catalog No. ABIN6252531

This Mouse Monoclonal antibody specifically detects ECM1 in IHC and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for ECM1 antibody (ABIN6252531)

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ECM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: SC05

Product Details anti-ECM1 Antibody

Purification: Protein G affinity chromatography

Immunogen: Secretory component protein isolated from human colostrum was used as the immunogen.

Isotype: IgG1 kappa

Application Details

Application Notes: IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for ECM1

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Alternative Name: Extracellular Matrix Protein 1

Background: This antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory component. It does not react with the cell lines lacking this protein. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Gene ID: 1893

UniProt: Q16610