Very Low Density Lipoprotein (VLDL) (C-Term) antibody

Catalog No. ABIN6252626

This anti- antibody is a Mouse Monoclonal antibody detecting in WB, FACS, IHC (p) and IF. Suitable for Human and Rat.

Quick Overview for Very Low Density Lipoprotein (VLDL) (C-Term) antibody (ABIN6252626)

Target: Very Low Density Lipoprotein (VLDL)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: VLDLR-1337

Product Details

Binding Specificity: C-Term

Purification: Protein G affinity chromatography

Immunogen: A human recombinant partial protein from the C-terminal region was used as the immunogen for this VLDL Receptor antibody.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 1-2 μg/mL

Western blot: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the VLDL Receptor antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details

Target: Very Low Density Lipoprotein (VLDL)

Alternative Name: VLDL

Background: Very Low Density Lipoprotein Receptor (VLDLR/VLDL receptor) is a member of the LDL receptor gene family which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR associates with RAP (receptor associated protein) during receptor folding, and RAP facilitates the secretion of the extracellular region of VLDLR. VLDL receptor is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein), which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development.

Gene ID: 7436

UniProt: P98155