MAML3 antibody

Catalog No. ABIN6252771

This Mouse Monoclonal antibody specifically detects MAML3 in FACS, IF and IHC. It exhibits reactivity toward Human.

Quick Overview for MAML3 antibody (ABIN6252771)

Target: MAML3 (Mastermind-Like 3 (MAML3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAML3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: MMLP3-1

Product Details anti-MAML3 Antibody

Purification: Protein G affinity chromatography

Immunogen: A human full length recombinant protein was used as the immunogen for this MAML3 antibody.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML3 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for MAML3

Target: MAML3 (Mastermind-Like 3 (MAML3))

Alternative Name: MAML3

Background: Mastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.

Gene ID: 55534

UniProt: Q96JK9