PAX7 antibody (AA 300-600)

Catalog No. ABIN6252801

This Mouse Monoclonal antibody specifically detects PAX7 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for PAX7 antibody (AA 300-600) (ABIN6252801)

Target: PAX7 (Paired Box 7 (PAX7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX7 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PBPX7

Product Details anti-PAX7 Antibody

Binding Specificity: AA 300-600

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment (aa300-600) was used as the immunogen for the PAX7 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the PAX7 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris buffer with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX7

Background: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

Gene ID: 5081

UniProt: P23759