CEA antibody

Catalog No. ABIN6252839

This anti-CEA antibody is a Mouse Monoclonal antibody detecting CEA in WB, IHC, FACS, ELISA and IF. Suitable for Human.

Quick Overview for CEA antibody (ABIN6252839)

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Clone: C66-1009

Product Details anti-CEA Antibody

Purification: Protein G affinity chromatography

Immunogen: Human full-length recombinant CEA protein was used as the immunogen for this antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: ELISA: order BSA-free format (3)

Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. Clone C66/1009 (recommended detect) will pair with clones < a href=../tds/cea-antibody-c661030-v2375>C66/1030 and < a href=../tds/cea-antibody-c66261-v2088>C66/261 (recommended capture) by ELISA.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Aliquot and Store at 2-8C. Avoid freez-thaw cycles.

Target Details for CEA

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Alternative Name: CEA

Background: This antibody recognizes proteins of 80-200  kDa, identified as different members of the CEA (Carcinoembryonic Antigen) family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. The antibody shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). CEA antibody positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Gene ID: 1048

UniProt: P06731