KCC2 antibody (pSer940)

Catalog No. ABIN6254065

Cited in 5+ publications. The Rabbit Polyclonal anti-KCC2 antibody (ABIN6254065) specifically detects KCC2 in WB, ELISA and IP. The antibody is reactive with Rat samples.

Quick Overview for KCC2 antibody (pSer940) (ABIN6254065)

Target: KCC2 (SLC12A5) (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 5 (SLC12A5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-KCC2 Antibody

Binding Specificity: pSer940

Specificity: Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Ser940. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: bovine, canine, human, mouse, non-human primate

Characteristics: Our Potassium Chloride Cotransporter (KCC2) (Ser940) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Potassium Chloride Cotransporter (KCC2) (Ser940) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IP.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: IP: 40 µL of antibody per 1 mg lysate

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-KCC2 antibody (ABIN6254065)

Kwon, Neupane, Shin, Gwon, Yin, Shin, Shin, Hong, Park, Yi, Kim, Kang: "Calpain-2 as a Treatment Target in Prenatal Stress-induced Epileptic Spasms in Infant Rats." in: Experimental neurobiology, Vol. 28, Issue 4, pp. 529-536, (2019) (PubMed).

Huang, Grau: "Ionic plasticity and pain: The loss of descending serotonergic fibers after spinal cord injury transforms how GABA affects pain." in: Experimental neurology, Vol. 306, pp. 105-116, (2019) (PubMed).

Mahadevan, Khademullah, Dargaei, Chevrier, Uvarov, Kwan, Bagshaw, Pawson, Emili, De Koninck, Anggono, Airaksinen, Woodin: "Native KCC2 interactome reveals PACSIN1 as a critical regulator of synaptic inhibition." in: eLife, Vol. 6, (2018) (PubMed).

Mahadevan, Dargaei, Ivakine, Hartmann, Ng, Chevrier, Ormond, Nothwang, McInnes, Woodin: "Neto2-null mice have impaired GABAergic inhibition and are susceptible to seizures." in: Frontiers in cellular neuroscience, Vol. 9, pp. 368, (2015) (PubMed).

Toda, Ishida, Kiyama, Yamashita, Lee: "Down-regulation of KCC2 expression and phosphorylation in motoneurons, and increases the number of in primary afferent projections to motoneurons in mice with post-stroke spasticity." in: PLoS ONE, Vol. 9, Issue 12, pp. e114328, (2015) (PubMed).

Target Details for KCC2

Target: KCC2 (SLC12A5) (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 5 (SLC12A5))

Alternative Name: KCC2

Background:

Synonyms: Electroneutral potassium chloride cotransporter 2 antibody, Electroneutral potassium-chloride cotransporter 2 antibody, Erythroid K Cl cotransporter 2 antibody, Furosemide sensitive K Cl cotransporter antibody, hKCC2 antibody, K-Cl cotransporter 2 antibody, KCC 2 antibody, KCC2 antibody, KIAA1176 antibody, Neuronal K Cl cotransporter antibody, Neuronal K-Cl cotransporter antibody, Potassium Chloride Cotransporter antibody, Potassium chloride transporter 5 antibody, rKCC2 antibody, S12A5 antibody, S12A5_HUMAN antibody, SLC12A5 antibody, Solute carrier family 12 (potassium chloride transporter) member 5 antibody, Solute carrier family 12 member 5 antibody

Description Logic: Our Potassium Chloride Cotransporter (KCC2) (Ser940) rabbit polyclonal phosphospecific primary antibody is produced . It detects Potassium Chloride Cotransporter (KCC2) (Ser940) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IP.

Gene Name Alternatives: KCC2

Molecular Weight: 135 kDa

Gene ID: 171373

UniProt: Q63633