SLC12A3 antibody (pThr53)

Catalog No. ABIN6254066

This Rabbit Polyclonal antibody specifically detects SLC12A3 in WB, IHC and ELISA. It exhibits reactivity toward Mouse.It has been mentioned in 1 publication

Quick Overview for SLC12A3 antibody (pThr53) (ABIN6254066)

Target: SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC12A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-SLC12A3 Antibody

Binding Specificity: pThr53

Specificity: Specific for endogenous levels of the ~160 kDa NCC protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-phosphatase.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: guinea pig, hamster

Characteristics: Our NCC (Thiazide sensitive NaCl cotransporter) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects NCC (Thiazide sensitive NaCl cotransporter) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of mouse NCC, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000-1:6000

Dilution Range: WB: 1:1000-1:6000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-SLC12A3 antibody (ABIN6254066)

Duan, Gu, Xiao, Gao, Wu, Zhang, Meng, Wang, Zhang, Lin, Wang, Gu: "Norepinephrine-Induced Stimulation of Kir4.1/Kir5.1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule." in: Hypertension (Dallas, Tex. : 1979), Vol. 73, Issue 1, pp. 112-120, (2019) (PubMed).

Target Details for SLC12A3

Target: SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))

Alternative Name: SLC12A3

Background:

Synonyms: FLJ96318 antibody, Na Cl cotransporter antibody, Na Cl symporter antibody, Na-Cl symporter antibody, NaCl electroneutral thiazide sensitive cotransporter antibody, NCCT antibody, SLC12A3 antibody, S12A3_HUMAN antibody, slc12a3 antibody, Solute carrier family 12 (sodium/chloride transporters) member 3 antibody, Solute carrier family 12 member 3 antibody, Thiazide sensitive Na Cl cotransporter antibody, Thiazide sensitive sodium chloride cotransporter antibody, Thiazide-sensitive sodium-chloride cotransporter antibody, TSC antibody

Description Logic: Our NCC (Thiazide sensitive NaCl cotransporter) rabbit polyclonal phosphospecific primary antibody is produced . It detects NCC (Thiazide sensitive NaCl cotransporter) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Gene Name Alternatives: SLC12A3

Molecular Weight: 160 kDa

Gene ID: 20497

UniProt: P59158