DDIT4 antibody (pThr23, pThr25)

Catalog No. ABIN6254086

The Rabbit Polyclonal anti-DDIT4 antibody (ABIN6254086) specifically detects DDIT4 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for DDIT4 antibody (pThr23, pThr25) (ABIN6254086)

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDIT4 Antibody

Binding Specificity: pThr23, pThr25

Specificity: Specific for endogenous levels of the ~34 kDa REDD1 phosphorylated at Thr23/25. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Human

Predicted Reactivity: non-human primate

Characteristics: Our REDD1 (Thr23/25) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects REDD1 (Thr23/25) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr23/25 of human REDD1, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

Target Details for DDIT4

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Alternative Name: DDIT4

Background:

Synonyms: DDIT4 antibody, DDIT4_HUMAN antibody, Dig2 antibody, DNA damage inducible transcript 4 antibody, DNA damage inducible transcript 4 protein antibody, DNA damage-inducible transcript 4 protein antibody, FLJ20500 antibody, HIF 1 responsive protein RTP801 antibody, HIF 1 responsive RTP801 antibody, HIF-1 responsive protein RTP801 antibody, Protein regulated in development and DNA damage response 1 antibody, REDD-1 antibody, REDD1 antibody, RTP801 antibody

Description Logic: Our REDD1 (Thr23/25) rabbit polyclonal phosphospecific primary antibody is produced . It detects REDD1 (Thr23/25) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: DDIT4

Molecular Weight: 34 kDa

Gene ID: 54541

UniProt: Q9NX09

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process