VEGFA antibody (Isoform 6)

Catalog No. ABIN6254198

There is 1 publication for this product available. The Goat Polyclonal anti-VEGFA antibody is suitable to detect VEGFA in samples from Human, Rat, Mouse, Pig, Dog, Rabbit, Cow, Chicken, Sheep, Horse, Guinea Pig, Monkey, Cat, Donkey, Goat, Hamster and Avian. It has been validated for WB and IF.

Quick Overview for VEGFA antibody (Isoform 6) (ABIN6254198)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human, Rat, Mouse, Pig, Dog, Rabbit, Cow, Chicken, Sheep, Horse, Guinea Pig, Monkey, Cat, Donkey, Goat, Hamster, Avian

Host: Goat

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-VEGFA Antibody

Binding Specificity: Isoform 6

Purpose: Anti-VEGFA

Sequence: MNFLLSWVHW SLALLLYLHH AKWSQAAPMA EGGGQNHHEV VKFMDVYQRS YCHPIETLVD IFQEYPDEIE YIFKPSCVPL MRCGGCCNDE GLECVPTEES NITMQIMRIK PHQGQHIGEM SFLQHNKCEC RPKKDRARQE KCDKPRR

Specificity: Detects endogenous levels of total VEGFA by Western blot in whole cell and tissue lysates.

Characteristics: Vascular endothelial growth factor A

Purification: Epitope affinity purified

Immunogen: Purified recombinant human VEGFA isoform 6 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:250-1:2,000, IF:1:50-1:250

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-VEGFA antibody (ABIN6254198)

Rodrigues, Matafome, Sereno, Almeida, Castelhano, Gamas, Neves, Gonçalves, Carvalho, Arslanagic, Wilcken, Fonseca, Simões, Conde, Castelo-Branco, Seiça: "Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance." in: Scientific reports, Vol. 7, Issue 1, pp. 1698, (2018) (PubMed).

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA

Background:

Goat polyclonal to VEGFA. This protein is a member of the PDGF/VEGF growth factor family. It is a glycosylated mitogen that specifically acts on endothelial cells and has various effects such as mediating increased vascular permeability, endothelial cell growth, promoting cell migration, inducing angiogenesis and inhibiting apoptosis.

MVCD1, RP1-261G23.1, VEGF, vascular permeability factor and VPF antibody.

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling