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C-Peptide antibody

The Goat Polyclonal anti-C-Peptide antibody has been validated for WB. It is suitable to detect C-Peptide in samples from Human and Rat.
Catalog No. ABIN6254201

Quick Overview for C-Peptide antibody (ABIN6254201)

Target

See all C-Peptide Antibodies
C-Peptide

Reactivity

  • 35
  • 21
  • 4
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  • 2
Human, Rat

Host

  • 44
  • 18
  • 1
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Goat

Clonality

  • 45
  • 19
Polyclonal

Conjugate

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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
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  • 1
This C-Peptide antibody is un-conjugated

Application

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Western Blotting (WB)
  • Sequence

    EAEDLQVGQV ELGGGPGAGS LQPLALEGSL QGT-EVEDPQVAQL ELGGGPGAGD LQTLALEVAR Q

    Specificity

    Detects C-peptide using MBP-C-peptide recombinant fusion protein and GFP-C-peptide expressed in transfected mammalian cells.

    Characteristics

    pro-insulin

    Purification

    This antibody is epitope-affinity purified from goat antiserum.

    Immunogen

    Purified recombinant human and rat C-peptides produced in E. coli as a fusion protein.

    Isotype

    IgG
  • Application Notes

    WB 1:500-1:2,000

    Restrictions

    For Research Use only
  • Concentration

    4.00 mg/mL

    Buffer

    Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.

    Handling Advice

    The antibody solution should be gently mixed before use.

    Storage

    4 °C,-20 °C

    Storage Comment

    For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.
  • Target

    C-Peptide

    Background

    C-Peptide is part of proinsulin, that consists of C-Peptide, alpha and beta chains that later become linked together to form the insulin molecule. Each proinsulin molecule produces one molecule of insulin and one molecule of C-peptide that is released into the blood. C-peptide stimulates the N+, K+ -ATPase and endothelial nitric oxide synthase activities by binding to cell membranes which leads to increased intracellular Ca2+ concentration.

    Gene ID

    3630
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