C-Peptide antibody

Catalog No. ABIN94267

Product Details anti-C-Peptide Antibody

Target: C-Peptide

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C-Peptide antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Specificity: The mouse monoclonal antibody C-PEP-01 reacts specifically with C-peptide, a part of the proinsulin molecule. Proinsulin consists of three parts: C-peptide and two long strands of amino acids (alpha and beta chains, later become linked together to form the insulin molecule). No cross-reactivity with insulin or other peptide hormones or proteins was observed.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human C-peptide conjugated to bovine serum albumin.

Clone: C-PEP-01

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 2-5 μg/mL, positive control: human pancreas (islets of Langerhans).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-C-Peptide antibody (ABIN94267)

Koblas, Zacharovova, Berkova, Girman, Saudek: "An acidic pH and activation of phosphoinositide 3-kinase stimulate differentiation of pancreatic progenitors into insulin-producing cells." in: Transplantation proceedings, Vol. 42, Issue 6, pp. 2075-80, (2010) (PubMed).

Hilgert, Stolba, Kristofová, Stefanová, Bendlová, Lebl, Horejsí: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." in: Hybridoma, Vol. 10, Issue 3, pp. 379-86, (1991) (PubMed).

Target Details for C-Peptide

Target: C-Peptide

Abstract: C-Peptide Products

Synonyms: insulin 2 antibody, Ins2 antibody

Background: From every molecule of proinsulin, one molecule of insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to insulin.,C-peptide of proinsulin