C-Peptide antibody

Details for Product No. ABIN94267
Antigen
Reactivity
Human
105
36
14
7
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Host
Mouse
113
33
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
15
7
6
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), ELISA
101
49
19
17
13
11
7
6
6
6
6
6
5
2
2
1
1
1
1
1
1
Options
Immunogen Human C-peptide conjugated to bovine serum albumin.
Clone C-PEP-01
Isotype IgG1
Specificity The antibody C-PEP-01 reacts specifically with C-peptide, a part of the Proinsulin molecule. Proinsulin consists of the three parts: C-peptide and two long strands of amino acids (alpha and beta chains, later become linked together to form the Insulin molecule).
No cross-reactivity with Insulin or other peptide hormones or proteins was observed.
Cross-Reactivity (Details) Human
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Background From every molecule of Proinsulin, one molecule of Insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to Insulin.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-C-Peptide antibody (ABIN94267) Immunohistochemistry of paraffin-embedded sections (human pancreas) Immunohistochemis...
Product cited in: Koblas, Zacharovova, Berkova, Girman, Saudek: "An acidic pH and activation of phosphoinositide 3-kinase stimulate differentiation of pancreatic progenitors into insulin-producing cells." in: Transplantation proceedings, Vol. 42, Issue 6, pp. 2075-80, 2010 (PubMed).

Hilgert, Stolba, Kristofová, Stefanová, Bendlová, Lebl, Horejsí: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." in: Hybridoma, Vol. 10, Issue 3, pp. 379-86, 1991 (PubMed).