YFP antibody

Catalog No. ABIN6254248

This Goat Polyclonal antibody specifically detects YFP in WB, IEM, IF, IHC (fro) and IHC (p). It exhibits reactivity toward Aequorea victoria.

Quick Overview for YFP antibody (ABIN6254248)

Target: YFP (Yellow Fluorescent Protein (YFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This YFP antibody is un-conjugated

Application: Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-YFP Antibody

Purpose: anti-YFP

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes GFP and does not recognize mCherry/red fluorescent protein.

Cross-Reactivity (Details): YFP, GFP, EGFP, Venus

Characteristics: Yellow Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IF:1:50-1:1,000, IHC-P:1:50-1:1,000, IHC-F:1:50-1:1,000, IEM:1:50-1:1,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for YFP

Target: YFP (Yellow Fluorescent Protein (YFP))

Alternative Name: YFP

Target Type: Synthetic

Background:

Goat polyclonal antibody to YFP (yellow fluorescent protein). YFP is a genetic mutant of green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 514/527 nm respectively.

Yellow fluorescent protein antibody.