Histone H4 antibody (meArg3)

Catalog No. ABIN6254877

The Rabbit Polyclonal anti-Histone H4 antibody has been validated for WB, IF, ELISA, IP, ChIP and ICC. It is suitable to detect Histone H4 in samples from Human, Mouse and Rat.

Quick Overview for Histone H4 antibody (meArg3) (ABIN6254877)

Target: Histone H4

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)

Product Details anti-Histone H4 Antibody

Binding Specificity: meArg3

Specificity: Histone H4R3me2a Antibody detects endogenous levels of total Histone H4R3me2a

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IF 1:50-1:200 IP 1:50-1:100 CHIP 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for Histone H4

Target: Histone H4

Background:

Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene: HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4

Molecular Weight: 11kDa

Gene ID: 121, 504, 554

UniProt: P62805