Histone H4 antibody (acLys5)

Catalog No. ABIN6254903

This Rabbit Polyclonal antibody specifically detects Histone H4 in WB, IHC, IF, ELISA and ICC. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for Histone H4 antibody (acLys5) (ABIN6254903)

Target: Histone H4

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-Histone H4 Antibody

Binding Specificity: acLys5

Specificity: Acetyl-Histone H4 (Lys5) Antibody detects endogenous levels of Acetyl-Histone H4 only when phosphorylated at Lys5

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Immunogen affinity purified

Immunogen: A synthesized peptide derived from human Acetyl-Histone H4 around the phosphorylation site of Lys5

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-Histone H4 antibody (ABIN6254903)

Hu, Chang, Yan, Rong, Yang, Cheng, Gu, Yao, Hou, Mo, Zhao, Chen, Dinlin, Wang, Fang: "(-)-Epigallocatechin-3-gallate induces cancer cell apoptosis via acetylation of amyloid precursor protein." in: Medical oncology (Northwood, London, England), Vol. 32, Issue 1, pp. 390, (2016) (PubMed).

Target Details for Histone H4

Target: Histone H4

Background:

Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene: HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4

Molecular Weight: 11kDa

Gene ID: 121, 504, 554

UniProt: P62805