APC antibody (pSer2054)

Catalog No. ABIN6255083

This Rabbit Polyclonal antibody specifically detects APC in WB, IHC, ELISA, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for APC antibody (pSer2054) (ABIN6255083)

Target: APC (Adenomatous Polyposis Coli (APC))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-APC Antibody

Binding Specificity: pSer2054

Specificity: Phospho-APC (Ser2054) Antibody detects endogenous levels of APC only when phosphorylated at Sersine 2054.

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human APC around the phosphorylation site of Ser2054.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: APC

Background:

Description: Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Gene: APC

Molecular Weight: 311kDa

Gene ID: 324

UniProt: P25054

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization