EIF4B antibody (pSer422)

Catalog No. ABIN6255321

The Rabbit Polyclonal anti-EIF4B antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect EIF4B in samples from Human and Mouse.

Quick Overview for EIF4B antibody (pSer422) (ABIN6255321)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-EIF4B Antibody

Binding Specificity: pSer422

Specificity: Phospho-eIF4B (Ser422) Antibody detects endogenous levels of eIF4B only when phosphorylated at Ser422.

Predicted Reactivity: Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: EIF4B

Background:

Description: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Gene: EIF4B

Molecular Weight: 69 kDa

Gene ID: 1975

UniProt: P23588