ATR antibody (pSer428)

Catalog No. ABIN6255330

The Rabbit Polyclonal anti-ATR antibody is suitable to detect ATR in samples from Human, Mouse and Rat. It has been validated for ELISA, WB and IHC.

Quick Overview for ATR antibody (pSer428) (ABIN6255330)

Target: ATR (ATR serine/threonine kinase (ATR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATR antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATR Antibody

Binding Specificity: pSer428

Specificity: Phospho -ATR(S428) Antibody detects endogenous levels of ATR only when phosphorylated at S428

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human phospho -ATR(S428)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000 IHC 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for ATR

Target: ATR (ATR serine/threonine kinase (ATR))

Alternative Name: ATR

Background:

Description: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

Gene: ATR

Molecular Weight: 300 kDa

Gene ID: 545

UniProt: Q13535

Pathways: Positive Regulation of Response to DNA Damage Stimulus