alpha Adducin antibody (pSer726)

Catalog No. ABIN6255485

This Rabbit Polyclonal antibody specifically detects alpha Adducin in WB, IHC, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for alpha Adducin antibody (pSer726) (ABIN6255485)

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Adducin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-alpha Adducin Antibody

Binding Specificity: pSer726

Specificity: Phospho-ADD1 (Ser726) Antibody detects endogenous levels of ADD1 only when phosphorylated at Serine 726.

Predicted Reactivity: Zebrafish,Bovine,Horse,Dog,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for alpha Adducin

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Alternative Name: ADD1,ADD2

Background:

Description: Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Gene: ADD1

Molecular Weight: 80kDa

Gene ID: 118, 119

UniProt: P35611, P35612

Pathways: Negative Regulation of Hormone Secretion, Regulation of Actin Filament Polymerization, Regulation of Lipid Metabolism by PPARalpha, ER-Nucleus Signaling