SRF antibody (pSer99)

Catalog No. ABIN6255577

The Rabbit Polyclonal anti-SRF antibody (ABIN6255577) specifically detects SRF in WB, ELISA, IHC, ICC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SRF antibody (pSer99) (ABIN6255577)

Target: SRF (Serum Response Factor (SRF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-SRF Antibody

Binding Specificity: pSer99

Specificity: Phospho-SRF (Ser99) Antibody detects endogenous levels of SRF only when phosphorylated at Ser103, which site historically referenced as Ser99.

Predicted Reactivity: Pig,Bovine,Rabbit,Dog

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human SRF around the phosphorylation site of Ser99.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SRF

Target: SRF (Serum Response Factor (SRF))

Alternative Name: SRF

Background:

Description: SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.

Gene: SRF

Molecular Weight: 51kDa

Gene ID: 6722

UniProt: P11831