PPP1R12A antibody (Myosin Phosphatase, Target Subunit 1) (pThr853)

Details for Product anti-PPP1R12A Antibody No. ABIN6255746
Antigen
Epitope
pThr853
32
25
15
13
9
8
8
8
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
174
137
116
10
9
9
5
5
3
3
3
3
2
1
Host
Rabbit
172
1
1
Clonality
Polyclonal
Conjugate
This PPP1R12A antibody is un-conjugated
9
9
9
9
9
9
9
2
2
2
2
2
2
2
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
106
52
51
28
21
9
8
2
2
2
1
1
Options
Immunogen A synthesized peptide derived from human MYPT1 (Phospho-Thr853)
Isotype IgG
Specificity MYPT1 (Phospho-Thr853) Antibody detects endogenous levels of total MYPT1 (Phospho-Thr853)
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Antigen
Alternative Name MYPT1 (PPP1R12A Antibody Abstract)
Background

Description: Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Gene: PPP1R12A

Molecular Weight 115 kDa
Gene ID 4659
UniProt O14974
Pathways M Phase
Application Notes WB 1:500-1:2000,IHC 1:50-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr853) antibody (ABIN6255746) Western blot analysis of extracts of various smaple,using PMYPT1 (Phospho-Thr853) Ant...
Image no. 2 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr853) antibody (ABIN6255746) Western blot analysis of MYPT1 (Phospho-Thr853) expression in NIH/3T3 cell extract.Th...
Image no. 3 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr853) antibody (ABIN6255746) ABIN6268991 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr853) antibody (ABIN6255746) ABIN6268991 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was f...
Product cited in: Xiaojun, Yan, Hong, Xianghong, Shifeng, Dingjie, Xuemin, Lijuan, Bonan, Zhongqiu, Ruimin, Brann, Fang: "Acetylated α-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline(Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica." in: Scientific reports, Vol. 6, pp. 32257, 2018 (PubMed).

Ye, Jing, Li, Wu, Li, Xu: "Icariin promotes proliferation and osteogenic differentiation of rat adipose-derived stem cells by activating the RhoA-TAZ signaling pathway." in: Biomedicine & pharmacotherapy, Vol. 88, pp. 384-394, 2017 (PubMed).