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HSP27 antibody (pSer15)

The Rabbit Polyclonal anti-HSP27 antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect HSP27 in samples from Human, Mouse and Rat. There is 1 publication available.
Catalog No. ABIN6256149

Quick Overview for HSP27 antibody (pSer15) (ABIN6256149)

Target

See all HSP27 (HSPB1) Antibodies
HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity

  • 320
  • 103
  • 90
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  • 13
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  • 5
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Human, Mouse, Rat

Host

  • 250
  • 83
Rabbit

Clonality

  • 214
  • 118
Polyclonal

Conjugate

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This HSP27 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Binding Specificity

    • 42
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    pSer15

    Specificity

    Phospho-HSP27 (Ser15) Antibody detects endogenous levels of HSP27 only when phosphorylated at Serine 15.

    Predicted Reactivity

    Pig,Bovine,Horse,Sheep,Rabbit,Dog

    Purification

    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

    Immunogen

    A synthesized peptide derived from human HSP27 around the phosphorylation site of Ser15.

    Isotype

    IgG
  • Application Notes

    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS, pH 7.4,50 % glycerol.

    Storage

    -20 °C

    Expiry Date

    12 months
  • Xia, Yao, Tang, Xiao, Yang, Zhou: "Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Proteomic Analysis of Hugan Qingzhi and Its Protective Properties against Free Fatty Acid-Induced L02 Hepatocyte Injury." in: Frontiers in pharmacology, Vol. 8, pp. 99, (2017) (PubMed).

  • Target

    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

    Alternative Name

    HSPB1

    Background

    Description: Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

    Gene: HSPB1

    Molecular Weight

    27kDa

    Gene ID

    3315

    UniProt

    P04792

    Pathways

    MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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