HSP27 antibody (pSer15)
Quick Overview for HSP27 antibody (pSer15) (ABIN6256149)
Target
See all HSP27 (HSPB1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pSer15
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Specificity
- Phospho-HSP27 (Ser15) Antibody detects endogenous levels of HSP27 only when phosphorylated at Serine 15.
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Predicted Reactivity
- Pig,Bovine,Horse,Sheep,Rabbit,Dog
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Purification
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
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Immunogen
- A synthesized peptide derived from human HSP27 around the phosphorylation site of Ser15.
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Isotype
- IgG
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Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- PBS, pH 7.4,50 % glycerol.
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Storage
- -20 °C
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Expiry Date
- 12 months
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Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Proteomic Analysis of Hugan Qingzhi and Its Protective Properties against Free Fatty Acid-Induced L02 Hepatocyte Injury." in: Frontiers in pharmacology, Vol. 8, pp. 99, (2017) (PubMed).
: "
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Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Proteomic Analysis of Hugan Qingzhi and Its Protective Properties against Free Fatty Acid-Induced L02 Hepatocyte Injury." in: Frontiers in pharmacology, Vol. 8, pp. 99, (2017) (PubMed).
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- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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Alternative Name
- HSPB1
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Background
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Description: Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
Gene: HSPB1
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Molecular Weight
- 27kDa
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Gene ID
- 3315
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UniProt
- P04792
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Pathways
- MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
Target
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