LIPE antibody (pSer554, pSer855)

Catalog No. ABIN6256370

This Rabbit Polyclonal antibody specifically detects LIPE in WB, IHC, ELISA, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LIPE antibody (pSer554, pSer855) (ABIN6256370)

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIPE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-LIPE Antibody

Binding Specificity: pSer554, pSer855

Specificity: Phospho-HSL (Ser855/Ser554) Antibody detects endogenous levels of HSL only when phosphorylated at Ser855(Isoform 1) or Ser554(Isoform 2).

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human HSL around the phosphorylation site of Ser855/554.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for LIPE

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Alternative Name: LIPE

Background:

Description: In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

Gene: LIPE

Molecular Weight: 81+83kDa

Gene ID: 3991

UniProt: Q05469

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism