Histone H2A antibody (pThr121)

Catalog No. ABIN6256470

The Rabbit Polyclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Human, Rat and Mouse. It has been validated for WB, IHC and ELISA.

Quick Overview for Histone H2A antibody (pThr121) (ABIN6256470)

Target: Histone H2A

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Histone H2A Antibody

Binding Specificity: pThr121

Specificity: Phospho-Histone H2A (Thr121) Antibody detects endogenous levels of Histone H2A only when phosphorylated at Threonine 121

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Histone H2A around the phosphorylation site of Threonine 121

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:200 WB 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for Histone H2A

Target: Histone H2A

Background:

Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene: HIST1H2AG, HIST1H2AI, HIST1H2AK, HIST1H2AL, HIST1H2AM

Molecular Weight: 15kDa

Gene ID: 83, 298, 330

UniProt: P0C0S8, P04908, P20671, Q96KK5, Q6FI13, Q7L7L0