B-Cell Linker antibody (pTyr96)

Catalog No. ABIN6256814

This Rabbit Polyclonal antibody specifically detects B-Cell Linker in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for B-Cell Linker antibody (pTyr96) (ABIN6256814)

Target: B-Cell Linker (BLNK)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B-Cell Linker antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-B-Cell Linker Antibody

Binding Specificity: pTyr96

Specificity: Phospho-BLNK (Tyr96) Antibody detects endogenous levels of BLNK only when phosphorylated at Tyrosine 96.

Predicted Reactivity: Pig,Zebrafish,Bovine,Sheep,Rabbit,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human BLNK around the phosphorylation site of Tyr96.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background:

Description: Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

Gene: BLNK

Molecular Weight: 50kDa

Gene ID: 29760

UniProt: Q8WV28

Pathways: BCR Signaling