CNOT7 antibody (C-Term)

Catalog No. ABIN6257033

This Rabbit Polyclonal antibody specifically detects CNOT7 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for CNOT7 antibody (C-Term) (ABIN6257033)

Target: CNOT7 (CCR4-NOT Transcription Complex, Subunit 7 (CNOT7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CNOT7 Antibody

Binding Specificity: C-Term

Specificity: CNOT7 Antibody detects endogenous levels of total CNOT7.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CNOT7, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CNOT7

Target: CNOT7 (CCR4-NOT Transcription Complex, Subunit 7 (CNOT7))

Alternative Name: CNOT7

Background:

Description: Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.

Gene: CNOT7

Molecular Weight: 36 kDa

Gene ID: 29883

UniProt: Q9UIV1

Pathways: Ribonucleoprotein Complex Subunit Organization