E2F7 antibody

Catalog No. ABIN6257151

The Rabbit Polyclonal anti-E2F7 antibody is suitable to detect E2F7 in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for E2F7 antibody (ABIN6257151)

Target: E2F7 (E2F Transcription Factor 7 (E2F7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-E2F7 Antibody

Specificity: E2F7 antibody detects endogenous levels of total E2F7

Cross-Reactivity: Human

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for E2F7

Target: E2F7 (E2F Transcription Factor 7 (E2F7))

Alternative Name: E2F7

Background:

Description: Atypical E2F transcription factor that participates in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1. Acts as a regulator of S-phase by recognizing and binding the E2-related site 5'-TTCCCGCC-3' and mediating repression of G1/S-regulated genes. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Also involved in DNA damage response: up-regulated by p53/TP53 following genotoxic stress and acts as a downstream effector of p53/TP53-dependent repression by mediating repression of indirect p53/TP53 target genes involved in DNA replication. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene. Acts as a negative regulator of keratinocyte differentiation.

Gene: E2F7

Molecular Weight: 99 kDa

Gene ID: 144455

UniProt: Q96AV8