POU4F3 antibody (Internal Region)

Catalog No. ABIN6257424

The Rabbit Polyclonal anti-POU4F3 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect POU4F3 in samples from Human, Mouse and Rat.

Quick Overview for POU4F3 antibody (Internal Region) (ABIN6257424)

Target: POU4F3 (POU Domain, Class 4, Transcription Factor 3 (POU4F3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU4F3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POU4F3 Antibody

Binding Specificity: Internal Region

Specificity: POU4F3 Antibody detects endogenous levels of total POU4F3.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POU4F3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for POU4F3

Target: POU4F3 (POU Domain, Class 4, Transcription Factor 3 (POU4F3))

Alternative Name: POU4F3

Background:

Description: Acts as a transcriptional activator (PubMed:18228599). Acts by binding to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes (PubMed:18228599). Involved in the auditory system development, required for terminal differentiation of hair cells in the inner ear (By similarity).

Gene: POU4F3

Molecular Weight: 35 kDa

Gene ID: 5459

UniProt: Q15319

Pathways: Sensory Perception of Sound