PPP4R2 antibody (Internal Region)

Catalog No. ABIN6257426

This Rabbit Polyclonal antibody specifically detects PPP4R2 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for PPP4R2 antibody (Internal Region) (ABIN6257426)

Target: PPP4R2 (Protein Phosphatase 4, Regulatory Subunit 2 (PPP4R2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP4R2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PPP4R2 Antibody

Binding Specificity: Internal Region

Specificity: PPP4R2 Antibody detects endogenous levels of total PPP4R2.

Predicted Reactivity: Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PPP4R2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PPP4R2

Target: PPP4R2 (Protein Phosphatase 4, Regulatory Subunit 2 (PPP4R2))

Alternative Name: PPP4R2

Background:

Description: Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.

Gene: PPP4R2

Molecular Weight: 50 kDa

Gene ID: 151987

UniProt: Q9NY27