RAD18 antibody (Internal Region)

Catalog No. ABIN6257452

This anti-RAD18 antibody is a Rabbit Polyclonal antibody detecting RAD18 in WB, ELISA, IF and ICC. Suitable for Human and Rat.

Quick Overview for RAD18 antibody (Internal Region) (ABIN6257452)

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RAD18 Antibody

Binding Specificity: Internal Region

Specificity: RAD18 Antibody detects endogenous levels of total RAD18.

Predicted Reactivity: Pig,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RAD18, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Alternative Name: RAD18

Background:

Description: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.

Gene: RAD18

Molecular Weight: 56 kDa

Gene ID: 56852

UniProt: Q9NS91